Polydispersity of glycosaminoglycans in normal human urine.
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چکیده
منابع مشابه
Excretion patterns of glycosaminoglycans and glycoproteins in normal human urine.
Glycosaminoglycans and glycoproteins in the urine of 100 healthy, active, human subjects were examined by cellulose acetate electrophoresis and salt gradient, ion-exchange, column chromatography. The cetylpyridinium chloride (CPC) turbidity and uronic acid:creatinine ratio was also studied. Fractions were identified by electrophoretic mobility, staining reactions, susceptibility to enzyme diges...
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Certain of the physiochemical and immunochemical properties of the y-globulins of normal human urine have been reported by Webb, Rose, and Sehon (2) and by Franklin (3). These urinary proteins were found to be electrophoretically and antigenically closely related to serum y-globulins, but smaller in size; their molecular weights ranged from 10,600 (2) to 38,000 (3). Sedimentation coefficients S...
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Glycosaminoglycans have been characterized from a normal human breast cell line (HBL-100) and two different cell lines from human breast carcinoma (MDA-MB-231 and MCF-7). The glycosaminoglycans were labeled by exposure of cell cultures to [3H]glucosamine and [35S]sulfate and then isolated from both spent media and cells by pronase digestion and cetylpyridinium chloride fractionation. They were ...
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There is abundant evidence that glycosaminoglyeans are linked covalently to the protein in tissues (Mathews & Lozaityte, 1958; Muir, 1958). This fact suggested that isolation and characterization of glycosaminoglycan-peptides, particularly uronic acid-containing glycopeptides in normal urine, will assist in the understanding of the normal metabolism of glycosaminoglycans in tissues. The present...
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The fluorescence technique described here utilizes the electrostatic interaction between the polyanionic sites of glycosaminoglycans and the cationic dye Acridine Orange to analyse urinary glycosaminoglycans from patients suffering from mucopolysaccharidoses. The basis of the titration is the decrease in the fluorescence of free Acridine Orange that occurs when it is bound to polyanions. The ef...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1980
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.131.327